Antibodies against human influenza viruses in Galliformes order
نویسندگان
چکیده
Influenza A virus is always concerned as an infectious agent in birds and other mammalian such as humans. Although it is believed that barriers among various species restrict the transmission of influenza viruses, there are evidences implying that origin of all human influenza viruses is derived from avian influenza viruses. To determine the prevalence of human H1 and H3 viruses in the captive birds of Galliformes order, a serological surveillance was carried in Tehran Zoo, Saiee Park and Pardisan Park of Tehran, from November 2008 to February 2009. Sera samples were collected from 7 species including Chickens, Guinea fowls, Partridges, Pheasants, Turkeys and Quails and presence of antibodies was detected by haemagglutination inhibition assay. Sera of chickens immunized by human influenza vaccine were used as positive control in the assays. In total, 84.61% and 100% of sera samples had antibodies against human influenza H1 and H3 viruses, respectively. The Geometric Mean Titer (GMT) value for H1 antibodies was 30.33 whereas that related for H3 antibodies was 57.36. Significantly the highest GMT value and the greatest antibody titers were observed in chicken species. As HI assay is able to detect haemagglutination antibodies as soon as 2 weeks to 1 year post-infection, the results of this study indicate that seropositive captive birds were infected during recent year with H1 and H3 virus strain, closely related to human strains. Moreover, as the peak of influenza epidemic in human population in Tehran was at the same time with our sample collection, it could be concluded that influenza viruses’ transmission between human and captive birds occurred. Author details Influenza Unit, Pasteur Institute of Iran, Tehran, Iran. Department of Microbiology, Faculty of Biological Sciences, Shahid-Beheshti University, Tehran, Iran. Department of Clinical Sciences, Faculty of Specialized Veterinary Science, Science and Research Campus, Islamic Azad University, Tehran, Iran.
منابع مشابه
Evidence of H1 and H3 Influenza Virus Infection in Captive Birds in Tehran from November 2008 to February 2009
Background and Aims: Influenza A virus infects birds and some mammalian including human. H1 and H3 subtypes are circulated in both human and birds’ population. To determine the prevalence of the mentioned subtypes in birds, different captive bird species in Tehran zoo, and affiliated centers in Tehran were investigated for virus infection. Methods: In this study, Between November 2008 and...
متن کاملA Sensitive Neutralization Assay for Influenza C Viruses Based on the Acetylesterase Activity HEF Glycoprotein
Influenza C virus possesses specific neuraminate-O-acetylesterase as a receptor-destroying function. This enzymatic activity of the viral glycoprotein HEF (Hemagglutinin, esterase activity and fusion factor) can be visualized in situ by the use of distinct color substrates. Hereby the localization, as well as the quantity of synthesized HEF protein is detectable. We further developed the estera...
متن کاملSeasonal Outbreak of Influenza A virus Infection in Pediatric Age Groups During 2004-2005 in South of Iran
Background: The pandemic and regional influenza outbreaks resulting from antigenic variation of influenza viruses have been the subject of numerous studies which are crucial to the preparation of the vaccine. Frequent global winter outbreaks of influenza viruses require a constant surveillance of emerging influenza variants in order to develop efficient influenza vaccine. Methods: This study wa...
متن کاملMolecular Identification of Pre-Existing Immunityin Human against H9N2 Influenza Viruses Using HLA-A*0201 Binding Peptides
Background and Aims: The contribution genetic and antigenic diversity of H9N2 influenza viruses in evading from immune responses, cytotoxic T lymphocytes (CTL) epitopes in hemagglutinin (HA) protein restricted by HLA binding peptides was identified. Materials and Methods: Phylogenetic analyses were carried out for all of full length HA and deduced amino acid sequences of H9N2 viruses available ...
متن کاملHeamagglutinin Conserved Domain (HA2) Prepared in Prokaryotic System is Immunogenic in Mice but not Protective against Lethal Influenza Challenge
Background and Aims: Influenza vaccine production process is time-consuming with little-to-no cross-protection which requires annual adjustment. The construction of a universal vaccine to deal with the pandemics and epidemics which occasionally threat human population is the aim of many researches worldwide. Today, influenza vaccines are mostly against two major antigenic proteins, hemagglutini...
متن کامل